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nfdm  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology nfdm
    Nfdm, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 21302 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nfdm/Tween-20/bio_rxiv__2025__09__10__675329-90-6-27
    Average 96 stars, based on 21302 article reviews
    nfdm - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Mechanical instability of adherens junctions overrides intrinsic quiescence of hair follicle stem cells.
    Article Snippet: The protein concentration was estimated using a BCA kit (Promega). .. The blots were blocked using 5% NFDM (Santa Cruz) and incubated with primary antibody overnight at 4 C. The blots were washed with 13 TBST and incubated with HRP conjugated secondary antibodies (1:3000, CST) for 1h at RT. ..

    Article Title: Human CDK5–cyclin B1 structures uncover a conserved mitotic CDK activation mechanism
    Article Snippet: Denatured extracts were resolved on precast NuPAGE 4-12%, 1.5 mm Bis-Tris polyacrylamide gels (Life Technologies, NP0335) and transferred to 0.2 μm nitrocellulose membranes (BioRad, 1620112). .. The membranes were blocked in 5% NFDM (Non Fat Dry Milk, Research Product International, M17200) in TBS-1% Tween-20 for 1 hour and incubated in primary antibodies (Vinculin, Santa Cruz Biotechnology sc-25336, 1: 200; CDK1/cdc2, Cell Signaling Technology 28439, 1:1000; CDK2, Abcam ab32147, 1:1000; CDK5, Abcam ab40773, 1:2000; Cyclin A2: rabbit Novus Biologicals NBP131330, 1:500; Cyclin B1: rabbit Cell Signaling Technology 4138, 1:1000; H3pS10: rabbit, Cell Signaling Technology 9701, 1:500; CDK1 pT14/pY15: rabbit, Thermo Fisher Scientific 44-686G, 1:1000; CDK5 pY15: rabbit, Cell Signaling Technology 19051, 1:1000) at 4 °C for 16–18 hours. ..

    Fat:

    Article Title: Human CDK5–cyclin B1 structures uncover a conserved mitotic CDK activation mechanism
    Article Snippet: Denatured extracts were resolved on precast NuPAGE 4-12%, 1.5 mm Bis-Tris polyacrylamide gels (Life Technologies, NP0335) and transferred to 0.2 μm nitrocellulose membranes (BioRad, 1620112). .. The membranes were blocked in 5% NFDM (Non Fat Dry Milk, Research Product International, M17200) in TBS-1% Tween-20 for 1 hour and incubated in primary antibodies (Vinculin, Santa Cruz Biotechnology sc-25336, 1: 200; CDK1/cdc2, Cell Signaling Technology 28439, 1:1000; CDK2, Abcam ab32147, 1:1000; CDK5, Abcam ab40773, 1:2000; Cyclin A2: rabbit Novus Biologicals NBP131330, 1:500; Cyclin B1: rabbit Cell Signaling Technology 4138, 1:1000; H3pS10: rabbit, Cell Signaling Technology 9701, 1:500; CDK1 pT14/pY15: rabbit, Thermo Fisher Scientific 44-686G, 1:1000; CDK5 pY15: rabbit, Cell Signaling Technology 19051, 1:1000) at 4 °C for 16–18 hours. ..

    Milk:

    Article Title: Human CDK5–cyclin B1 structures uncover a conserved mitotic CDK activation mechanism
    Article Snippet: Denatured extracts were resolved on precast NuPAGE 4-12%, 1.5 mm Bis-Tris polyacrylamide gels (Life Technologies, NP0335) and transferred to 0.2 μm nitrocellulose membranes (BioRad, 1620112). .. The membranes were blocked in 5% NFDM (Non Fat Dry Milk, Research Product International, M17200) in TBS-1% Tween-20 for 1 hour and incubated in primary antibodies (Vinculin, Santa Cruz Biotechnology sc-25336, 1: 200; CDK1/cdc2, Cell Signaling Technology 28439, 1:1000; CDK2, Abcam ab32147, 1:1000; CDK5, Abcam ab40773, 1:2000; Cyclin A2: rabbit Novus Biologicals NBP131330, 1:500; Cyclin B1: rabbit Cell Signaling Technology 4138, 1:1000; H3pS10: rabbit, Cell Signaling Technology 9701, 1:500; CDK1 pT14/pY15: rabbit, Thermo Fisher Scientific 44-686G, 1:1000; CDK5 pY15: rabbit, Cell Signaling Technology 19051, 1:1000) at 4 °C for 16–18 hours. ..



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